DragonFly

MFS has developed a series of microfluidic cartridges for biological sample processing and detection. Examples include differential extraction cartridges, sample processing and amplification for biodefense applications, and clinical sample processing and detection. The microfluidic cartridge approach includes:

Advantages Low Cost Disposable Easy to Use Prevents sample to sample contamination Fast Results Portable Automated Prevents human error

Demonstrated Applications Environmental bacteria (e.g., E. coli) Biothreat agents Clinical samples (Human Papilloma Virus) Antibiotic resistant bacteria (e.g., MRSA) Forensics such as differential extraction

Examples of PositiveID’s Microfluidic Cartridges:

Differential Extraction Cartridge (Patent nos. 7,491,527, 7,553,647, and 7,553,647 B2). These patents are available for licensing.

MFSI’s Dragonfly Cartridge – provides cell lysis, nucleic acid purification, PCR amplification and fluorescent detection in less than 7 minutes on a 150 ul input sample. Proven applications include: environmental bacteria, human papilloma virus (HPV), and antibiotic resistant bacterial such as MRSA.

Gel verification results of the processing and amplification of a duplex HPV PCR assay from human cells on the Dragonfly cartridge. Processing and amplification time was fully automated and less than seven minutes.